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Calcium/Calmodulin-dependent
Protein Kinase Activates Serum Response Factor Transcription
Activity by Its Dissociation from Histone Deacetylase, HDAC4
Davis, F., Gupta, M., Camoretti-Mercado,
B., Schwartz, R., and Gupta, M. Calcium/Calmodulin-dependent
Protein Kinase Activates Serum Response Factor Transcription
Activity by Its Dissociation from Histone Deacetylase, HDAC4.
The Journal of
Biological Chemistry Vol. 278, Issue 22,
20047-20058, (2003). “Protein A/G-agarose
(20µl) was then added to each sample and incubated at
4 °C overnight on a rotating device.”
“…reactions were continued at room temperature
with continuous rocking for an additional 1 h.”
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Genomic
Analysis of Stationary-Phase and Exit in Saccharomyces cerevisiae:
Gene Expression and Identification of Novel Essential Genes
Martinez, M., et al. Genomic Analysis
of Stationary-Phase and Exit in Saccharomyces cerevisiae: Gene
Expression and Identification of Novel Essential Genes. Molecular
Biology of the Cell Vol. 15, 5295-5305
(2004). “Before microarray printing, …were
incubated at 21°C in 48-52% humidity for 4-20 h…”
“Slides were incubated in single-slide capacity
chambers…on a rotating platform at 42°C for 16-20
h.”
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Mechanical
Strain on Osteoblasts Activates Autophosphorylation of Focal
Adhesion Kinase and Proline-rich Tyrosine Kinase 2 Tyrosine
Sites Involved in ERK Activation
Boutahar, N., Guignandon, A., Vico, L., and Lafage-Proust, M.
Mechanical Strain on Osteoblasts Activates Autophosphorylation
of Focal Adhesion Kinase and Proline-rich Tyrosine Kinase 2
Tyrosine Sites Involved in ERK Activation. The
Journal of Biological Chemistry Vol. 279, Issue
29, 30588-30599, (2004) “After
incubation at 4 °C for 2 h on a rotating device, immune
complexes were precipitated at 4 °C overnight on a rotating
device by using protein-A Sepharose…”
“After transfer, membranes were blocked using 5%
nonfat dried milk in PBS, pH 7.4, and incubated overnight
at 4 °C with the …”
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Role
of bone marrow cell trafficking in replenishing skeletal muscle
SP and MP cell populations
Rivier,
F. et al. Role of bone marrow cell trafficking in replenishing
skeletal muscle SP and MP cell populations. Journal
of Cell Science Vol. 117, 1979-1988 (2004).
“Muscles were finely minced using sterile scalpels
and digested at 37°C in 1U ml-1 dispase (Roche) and 2
mg ml-1 collagenase-type 4 (Worthington) for 45 minutes using
an Enviro-Genie…”
“100 ng of denatured probe were added to each slide
and sections were hybridized overnight at 37°C in a humidified
chamber.”
“Slides were incubated in a humidified chamber
at 37°C for 30 minutes with digoxigenin blocking solution…
then for an additional 30 minutes with…”
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Screening
lambda cDNA or genomic libraries
Koelle,
M. 1997, “Screening lambda cDNA or genomic libraries”,
Yale University. Available from: http://info.med.yale.edu/mbb/koelle/protocols/protocol_lambda_library_sc.html.
“Add the probe to the blot/hyb. Solution, mix well,
and incubate overnight at 65° on a rocker…”
“Place on a rotating platform at room temp for
1-2 hours.”
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Structure
– function studies of human apolipoprotein A-V: a regulator
of plasma lipid homeostasis
U.S. Department of Energy Office
of Scientific & Technical Information. “Structure
– function studies of human apolipoprotein A-V: a regulator
of plasma lipid homeostasis” OSTI
ID 816380. Washington: GPO 2003. “Subsequently,
the IgG fraction was biotinylated by incubation for 15 min
with 5 ml 0.5 mg/ml sulfo-NHS-Ssbiotin… in borate buffer
on a rocking platform with slow agitation.”
“Non specific binding sites on the membrane were
blocked with 0.1% TTBS…overnight, at room temperature
while rotating.”
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Subtraction
of cap-trapped full-length cDNA libraries to select rare transcripts
Hirozane-Kishikawa,
T. et al. Subtraction of cap-trapped full-length cDNA libraries
to select rare transcripts. BioTechniques
Vol. 35, No. 3, 510-518 (2003). “One
microliter of 5 M NaCl was then added to the driver/tester
mixture, and the hybridization was performed at 4°C for
8 h…”
“The hybridized sample library was diluted with
500 µL HAP buffer, incubated at 65°C, and added
to the HAP, mixed vigorously, and rotated at 60°C for
30 min.”
“The HAP was washed twice with 500 µL HAP
buffer, rotating at 60°C for 30 min…”
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